Coding

Part:BBa_K2796027

Designed by: Shuangshuang Pu   Group: iGEM18_LZU-CHINA   (2018-10-04)


hSDC4-T2A-NadB

syndecan-4 (SDC4; supports budding of endosomal membranes to form multivesicular bodies), and a fragment of L-aspartate oxidase (NadB; possibly boosts cellular metabolism by tuning up the citric acid cycle18).We use T2A to ensure their coexpression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 192
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1080
    Illegal AgeI site found at 1700
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None